News & Events

When

Date - March 18, 2016
1:00 pm

What

Principal Scientist
Corporate Functions Analytical
The Procter & Gamble Company

“Comprehensive Identification and Quantitation of Constituents of Botanical Extracts for Safety Risk Assessments”

Abstract:

In order to support safety risk assessments that guide the need for more expensive in-vitro or in-vivo safety studies, we have developed a comprehensive approach to identify and quantitate the constituents of complex botanical mixtures.  The methodology utilizes separation by UHPLC, followed by UV, charged aerosol detection (CAD) and high resolution mass spectrometry (with MS/MS and MS³).  CAD provides quantitation for analytes where analytical reference standards are not available.  Quantitation is facilitated by the addition of an inverse gradient stream which compensates for the known sensitivity of the detector to mobile phase composition.  The identification of unknown compounds is accomplished using high resolution mass spectrometry to obtain molecular formulae coupled with database searching.  The value of orthogonal data (e.g., UV absorbance spectra) to aid compound identification will also be highlighted.