News & Events

When

Date - April 28, 2021
1:00 pm - 2:00 pm

What

Talk Title: Sequencing small non-coding RNA by using PANDORA-seq

Advisor: Dr. Norman Chiu

Abstract:

RNA sequencing is an indispensable technique for studying RNA biology and many other
related areas. However, despite of the recent advances in RNA sequencing including the
next generation sequencing technology, the resulting RNA sequences could be biased if the
sample of interests contained modified RNA. It is well established that RNA modifications
would interfere with the activity of reverse transcriptase, which is an essential component
in many RNA sequencing methods. In a recent report published in Nature, the authors
demonstrated a unique approach named PANDORA-seq could effectively overcome the
interferences from a number of RNA modifications. By using the new sequencing method,
they found the biodistribution of small non-coding RNA in various tissue types to be very
different from using the traditional sequencing method. Their results might have
implications for studying the functions of small non-coding RNA in the future.