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Ngoc Vu

Posted on November 9, 2016


Date - November 9, 2016
1:00 pm


Ph.D. Literature Seminar—751

Title: “Photochemical reaction coupled with tandem mass spectrometry for determination of C=C positions in lipids”


The biological function of lipid is determined by its molecular structure. Lipid molecules that differ in their fatty acyl C=C location can have very different biological functions.  Although the state-of- the-art mass spectrometry can help to elucidate lipid structure, such as head group and compositions of fatty acyl chains, no commercial instruments currently are capable of differentiating the lipid C=C positional isomers using simple tandem mass spectrometric techniques. Recent technique using photochemical Parterno-Buchi reaction coupled with mass spectrometer produces the dissociated fragment ions, which can pinpoint the C=C location in lipids. Furthermore, this technique has been applied to quantify the lipid C=C isomers in different tissue samples, as a result, C=C position specific-breast cancer biomarkers were identified.