Publications

2020

22. Chekan, J. R., Fallon, T. R., and Moore, B. S. (2020) Biosynthesis of marine toxins. Curr. Opin. Chem. Biol. 59, 119–129 (doi:10.1016/j.cbpa.2020.06.009)
21. Chekan, J. R., McKinnie, S. M. K., Noel, J. P., and Moore, B. S. (2020) Algal neurotoxin biosynthesis repurposes the terpene cyclase structural fold into an N -prenyltransferase. Proc. Natl. Acad. Sci. (doi:10.1073/pnas.2001325117)
20. Fiore, M. F., de Lima, S. T., Carmichael, W. W., McKinnie, S. M. K., Chekan, J. R., and Moore, B. S. (2020) Guanitoxin, re-naming a cyanobacterial organophosphate toxin. Harmful Algae. 92, 101737 (doi:10.1016/j.hal.2019.101737)

2019

19. Chekan, J. R., Ongpipattanakul, C., and Nair, S. K. (2019) Steric complementarity directs sequence promiscuous leader binding in RiPP biosynthesis. Proc. Natl. Acad. Sci. U. S. A. 116, 24049–24055 (doi:10.1073/pnas.1908364116)
18. Chekan, J. R., Lee, G. Y., El Gamal, A., Purdy, T. N., Houk, K. N., and Moore, B. S. (2019) Bacterial tetrabromopyrrole debrominase shares a reductive dehalogenation strategy with human thyroid deiodinase. Biochemistry. 58, 5329–5338 (doi:10.1021/acs.biochem.9b00318)
17. Chekan, J. R.*, Ongpipattanakul, C.*, Wright, T. R., Zhang, B., Bollinger, J. M., Rajakovich, L. J., Krebs, C., Cicchillo, R. M., and Nair, S. K. (2019) Molecular basis for enantioselective herbicide degradation imparted by aryloxyalkanoate dioxygenases in transgenic plants. Proc. Natl. Acad. Sci. 116, 13299–13304 (doi:10.1073/pnas.1900711116)
16. Chekan, J. R., McKinnie, S. M. K., Moore, M. L., Poplawski, S. G., Michael, T. P., and Moore, B. S. (2019) Scalable biosynthesis of the seaweed neurochemical, kainic acid. Angew. Chemie Int. Ed. 58, 8454–8457 (doi:10.1002/anie.201902910)
15. Luhavaya, H., Sigrist, R., Chekan, J. R., McKinnie, S. M. K., and Moore, B. S. (2019) Biosynthesis of L-4-chlorokynurenine, an antidepressant prodrug and a non-proteinogenic amino acid found in lipopeptide antibiotics. Angew. Chemie Int. Ed. 58, 8394–8399 (doi:10.1002/anie.201901571)

2018

14. Chekan, J. R., and Moore, B. S. (2018) Preparation and characterization of tetrabromopyrrole debrominase from marine proteobacteria. Methods Enzymol. 605, 253–65 (doi:10.1016/bs.mie.2018.01.031)
13. Brunson, J. K.*, McKinnie, S. M. K.*, Chekan, J. R., McCrow, J. P., Miles, Z. D., Bertrand, E. M., Bielinski, V. A., Luhavaya, H., Oborník, M., Smith, G. J., Hutchins, D. A., Allen, A. E., and Moore, B. S. (2018) Biosynthesis of the neurotoxin domoic acid in a bloom-forming diatom. Science. 361, 1356–8 (doi:10.1126/science.aau0382)
12. Chekan, J. R., and Moore, B. S. (2018) Biosynthesis of the antibiotic bicyclomycin in soil and pathogenic bacteria (Viewpoint). Biochemistry. 57, 897–898 (doi:10.1021/acs.biochem.7b01204)

2017

11. Chekan, J. R., Estrada, P., Covello, P. S., and Nair, S. K. (2017) Characterization of the macrocyclase involved in the biosynthesis of RiPP cyclic peptides in plants. Proc. Natl. Acad. Sci. U. S. A. 114, 6551–6556 (doi:10.1073/pnas.1620499114)
10. Repka, L. M.*, Chekan, J. R.*, Nair, S. K., and van der Donk, W. A. (2017) Mechanistic Understanding of Lanthipeptide Biosynthetic Enzymes. Chem. Rev. 117, 5457–5520 (doi:10.1021/acs.chemrev.6b00591)
9. Olivares, P.*, Ulrich, E. C.*, Chekan, J. R.*, van der Donk, W. A., and Nair, S. K. (2017) Characterization of Two Late-Stage Enzymes Involved in Fosfomycin Biosynthesis in Pseudomonads. ACS Chem. Biol. 12, 456–463 (doi:10.1021/acschembio.6b00939)

2016

8. Chekan, J. R., Koos, J. D., Zong, C., Maksimov, M. O., Link, A. J., and Nair, S. K. (2016) Structure of the lasso peptide isopeptidase identifies a topology for processing threaded substrates. J. Am. Chem. Soc. 138, 16452–16458 (doi:10.1021/jacs.6b10389)
7. Chekan, J. R.*, Cogan, D. P.*, and Nair, S. K. (2016) Molecular basis for resistance against phosphonate antibiotics and herbicides. Med. Chem. Commun. 7, 28–36 (doi:10.1039/C5MD00351B)

2015

6. Peck, S. C., Chekan, J. R., Ulrich, E. C., Nair, S. K., and van der Donk, W. A. (2015) A common late-stage intermediate in catalysis by 2-hydroxyethyl-phosphonate dioxygenase and methylphosphonate synthase. J. Am. Chem. Soc. 137, 3217–20 (doi:10.1021/jacs.5b00282)

2014

5. Chekan, J. R.*, Kwon, I. H.*, Agarwal, V.*, Dodd, D., Revindran, V., Mackie, R. I., Cann, I., and Nair, S. K. (2014) Structural and biochemical basis for mannan utilization by Caldanaerobius polysaccharolyticus strain ATCC BAA-17. J. Biol. Chem. 289, 34965–77 (doi:10.1074/jbc.M114.579904)
4. Zhang, M.*, Chekan, J. R.*, Dodd, D.*, Hong, P.-Y., Radlinski, L., Revindran, V., Nair, S. K., Mackie, R. I., and Cann, I. (2014) Xylan utilization in human gut commensal bacteria is orchestrated by unique modular organization of polysaccharide-degrading enzymes. Proc. Natl. Acad. Sci. U. S. A. 111, E3708–17 (doi:10.1073/pnas.1406156111)
3. Dunbar, K. L.*, Chekan, J. R.*, Cox, C. L., Burkhart, B. J., Nair, S. K., and Mitchell, D. A. (2014) Discovery of a new ATP-binding motif involved in peptidic azoline biosynthesis. Nat. Chem. Biol. 10, 823–9 (doi:10.1038/nchembio.1608)
2. Hung, J. E., Fogle, E. J., Garg, N., Chekan, J. R., Nair, S. K., and van der Donk, W. A. (2014) Chemical rescue and inhibition studies to determine the role of Arg301 in phosphite dehydrogenase. PLoS One. 9, e87134 (doi:10.1371/journal.pone.0087134)
1. Agarwal, V., Peck, S. C., Chen, J.-H., Borisova, S. A., Chekan, J. R., van der Donk, W. A., and Nair, S. K. (2014) Structure and function of phosphonoacetaldehyde dehydrogenase: the missing link in phosphonoacetate formation. Chem. Biol. 21, 125–35 (doi:10.1016/j.chembiol.2013.11.006)